Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=FNS(Oct,PGM) | ||
|description=[[File: | |description=[[File:ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama.png|200px]] | ||
'''[[ | |info='''B''' '''[[SUIT-003]]''' [[Stadlmann 2006 Cell Biochem Biophys]] | ||
|application=O2 | |||
}} | |||
::: '''[[SUIT protocol pattern]]:''' complex cell [[Coupling-control protocol]] ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama | |||
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[[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | |||
[[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | |||
== Coupling-control protocol == | |||
::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma | |||
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{| class="wikitable" border="1" | |||
|- | |||
! Step | |||
! Respiratory state | |||
! Pathway control | |||
! Comment | |||
'''[[ | |- | ||
| ce1 | |||
| [[ROUTINE]] | |||
| [[Living cells |Iiving cells (ce)]] | |||
| Dead cells (dce) without substrate are in a state of ROX. | |||
Β | |||
|- | |||
| ce2SD | |||
| ''R''+S<sub>''P''</sub> | |||
| [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | |||
| dce with functional mitochondria are in state S<sub>''P''</sub>. | |||
Β | |||
|- | |||
| ce3Omy | |||
| ''L''+S<sub>''L''</sub> | |||
| [[LEAK respiration]] (''L'') + [[S]] (S<sub>''L''</sub>) | |||
| ce and dce are in the LEAK state. | |||
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|- | |||
| ce4U | |||
| ''E''+S<sub>''E''</sub> | |||
| [[Electron transfer pathway]] (''E'') + [[S]] (S<sub>''E''</sub>) | |||
| ce and dce are in the ET state. | |||
Β | |||
|- | |||
| ce5Rot | |||
| ROX+S<sub>''E''</sub> | |||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | |||
| ce are in the ROX state, dce are in the ET state. | |||
Β | |||
|- | |||
| ce6Ama | |||
| ROX<sub>Ama</sub> | |||
| [[ROX]] | |||
| | |||
Β | |||
|} | |||
Β | |||
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:::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium''' | |||
:::: '''Living cells & succinate''': ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | |||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol, SUIT B | ||
}} | }} | ||
Latest revision as of 12:33, 25 November 2020
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: FNS(Oct,PGM)
Reference: B SUIT-003 Stadlmann 2006 Cell Biochem Biophys
O2k-Application: O2
- SUIT protocol pattern: complex cell Coupling-control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
Coupling-control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
ce1 | ROUTINE | Iiving cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
ce2SD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
ce3Omy | L+SL | LEAK respiration (L) + S (SL) | ce and dce are in the LEAK state. |
ce4U | E+SE | Electron transfer pathway (E) + S (SE) | ce and dce are in the ET state. |
ce5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
ce6Ama | ROXAma | ROX |
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts:
SUIT protocol,
SUIT B