Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=FNS(Oct,PGM) | |||
|description=[[File:ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama.png|200px]] | |description=[[File:ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama.png|200px]] | ||
|info='''B''' [[Stadlmann 2006 Cell Biochem Biophys]] | |info='''B''' [[Stadlmann 2006 Cell Biochem Biophys]] | ||
|application=O2 | |||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama | |||
::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] | |||
[[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | [[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | ||
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:::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium''' | :::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium''' | ||
:::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol, SUIT B | |||
}} | |||
Revision as of 09:47, 15 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: FNS(Oct,PGM)
Reference: B Stadlmann 2006 Cell Biochem Biophys
O2k-Application: O2
- SUIT protocol pattern: complex cell coupling control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
,3L(S),4E(S)-5ROX(S),6ROX.jpg)
Coupling control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| ce1 | ROUTINE | Intact cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
| ce2SD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
| ce3Omy | L+SL | LEAK (L) + S (SL) | ce and dce are in the LEAK state. |
| ce4U | E+SE | ET-pathway (E) + S (SE) | ce and dce are in the ET state. |
| ce5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
| ce6Ama | ROXAma | ROX |
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Intact cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts:
SUIT protocol,
SUIT B
