Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
From Bioblast
| Line 22: | Line 22: | ||
|- | |- | ||
| | | ce1 | ||
| [[ROUTINE]] | | [[ROUTINE]] | ||
| [[Intact cells |Intact cells (ce)]] | | [[Intact cells |Intact cells (ce)]] | ||
| Line 28: | Line 28: | ||
|- | |- | ||
| | | ce2SD | ||
| ''R''+S<sub>''P''</sub> | | ''R''+S<sub>''P''</sub> | ||
| [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | | [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | ||
| Line 34: | Line 34: | ||
|- | |- | ||
| | | ce3Omy | ||
| ''L''+S<sub>''L''</sub> | | ''L''+S<sub>''L''</sub> | ||
| [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | | [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | ||
| Line 40: | Line 40: | ||
|- | |- | ||
| | | ce4U | ||
| ''E''+S<sub>''E''</sub> | | ''E''+S<sub>''E''</sub> | ||
| [[ET-pathway]] (''E'') + [[S]] (S<sub>''E''</sub>) | | [[ET-pathway]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| Line 46: | Line 46: | ||
|- | |- | ||
| | | ce5Rot | ||
| ROX+S<sub>''E''</sub> | | ROX+S<sub>''E''</sub> | ||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | | [[ROX]]+[[S]] (S<sub>''E''</sub>) | ||
| Line 52: | Line 52: | ||
|- | |- | ||
| | | ce6Ama | ||
| ROX<sub>Ama</sub> | | ROX<sub>Ama</sub> | ||
| [[ROX]] | | [[ROX]] | ||
| Line 60: | Line 60: | ||
:::* ''' | :::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium''' | ||
:::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 14:45, 29 November 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT B
- SUIT protocol pattern: complex cell coupling control protocol (ceCCP)
,3L(S),4E(S)-5ROX(S),6ROX.jpg)
Coupling control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| ce1 | ROUTINE | Intact cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
| ce2SD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
| ce3Omy | L+SL | LEAK (L) + S (SL) | ce and dce are in the LEAK state. |
| ce4U | E+SE | ET-pathway (E) + S (SE) | ce and dce are in the ET state. |
| ce5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
| ce6Ama | ROXAma | ROX |
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Intact cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
