Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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Revision as of 17:30, 10 November 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: ceRLE(SD)
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT B
- SUIT-category: ceRLE(SD)
- SUIT protocol pattern: complex cell coupling control protocol (ceCCP)
,3L(S),4E(S)-5ROX(S),6ROX.jpg)
Coupling control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| 1ce | ROUTINE | Intact cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
| 2ceSD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
| 3ceOmy | L+SL | LEAK (L) + S (SL) | ce and dce are in the LEAK state. |
| 4ceU | E+SE | ET-pathway (E) + S (SE) | ce and dce are in the ET state. |
| 5ceRot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
| 6ceAma | ROXAma | ROX |
- 1ce;1ceS .. versus 1ce;2ceOmy;3ceU;4ceS .. and respiration medium
- Intact cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
