Pyruvate: Difference between revisions
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'''Preparation of 2 M Pyruvic acid solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 2 M Pyruvic acid solution''' (dissolved in H<sub>2</sub>O): | ||
::!!Prepare fresh every day!! | |||
::* weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube | ::* weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube | ||
Revision as of 11:04, 22 November 2010
Description
Oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase and yields acetyl-CoA.
Pyruvate is an anion which cannot permeate through the lipid bilayer of membranes and hence requires a carrier: pyruvate carrier.
Abbreviation: P
Reference: MiPNet11.04, MiPNet09.12
Labels:
Enzyme: TCA Cycle and Matrix Dehydrogenases"TCA Cycle and Matrix Dehydrogenases" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Application in HRR
P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na; Sigma P 2256 (R.T.): FW = 110.0)
Preparation of 2 M Pyruvic acid solution (dissolved in H2O):
- !!Prepare fresh every day!!
- weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube
- !!Prepare fresh every day!!
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 ยตl using a 25 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 5 mM