OctPGM pathway control state

high-resolution terminology - matching measurements at high-resolution

OctPGM pathway control state

Description

OctPGM: Octanoylcarnitine & Pyruvate & Glutamate & Malate.

MitoPathway control state: FN

SUIT protocols: SUIT-002

This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<1 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate-anaplerotic pathway control state, M alone), and pyruvate and glutamate is added to compare FAO as the background state with FN as the reference state.

Abbreviation: OctPGM

Reference: MiPNet21.06 SUIT RP

More details

» FN

» Additive effect of convergent electron flow

» Respiratory complexes - more than five

» OctGM

» Convergent electron flow

OctPGM_L

FN pathway in the LEAK state can be evaluated in the following SUIT protocols:

OctPGM_P

FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

• SUIT-002

• DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
• DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
• DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007

• SUIT-015

OctPGM_E

FN pathway in the ET state can be evaluated in the following SUIT protocols:

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MitoPedia concepts: SUIT state