OctM pathway control state: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=OctM | ||
|description=''' | |description='''OctM''': [[Octanoylcarnitine]] & [[Malate]]. | ||
'''MitoPathway control state:''' F | '''MitoPathway control state:''' F | ||
'''SUIT protocols:''' [[ | '''SUIT protocols:''' [[SUIT-002]], [[SUIT-015]], [[SUIT-016]], [[SUIT-017]] | ||
{{Template:SUIT F(N)}} {{Template:SUIT M_low}} {{Template:SUIT M_high}} | |||
= | |info=[[MiPNet21.06 SUIT RP]], [[SUIT reference protocol]] | ||
}} | |||
::: ''More details'' | |||
::::» [[Fatty acid oxidation pathway control state]] | |||
::: | |||
[[ | == OctM<sub>''L''</sub> == | ||
FAO in the LEAK state can be evaluated in the following SUIT protocols: | |||
:::*[[SUIT-005]] | |||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-005 O2 pfi D011]] | |||
:::*[[SUIT-015]] | |||
:::*[[SUIT-016]] | |||
:::*[[SUIT-017]] | |||
== OctM<sub>''P''</sub> == | |||
FAO in the OXPHOS state can be evaluated in the following SUIT protocols: | |||
:::*[[SUIT-002]] | |||
::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]] | |||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | |||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 ce-pce D007]] | |||
:::*[[SUIT-005]] | |||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-005 O2 pfi D011]] | |||
:::*[[SUIT-015]] | |||
:::*[[SUIT-016]] | |||
:::*[[SUIT-017]] | |||
== OctM<sub>''E''</sub> == | |||
FAO in the ET state can be evaluated in the following SUIT protocols: | |||
---- | ---- | ||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT state | |||
}} | |||
Latest revision as of 04:56, 19 July 2022
- high-resolution terminology - matching measurements at high-resolution
OctM pathway control state
Description
OctM: Octanoylcarnitine & Malate.
MitoPathway control state: F
SUIT protocols: SUIT-002, SUIT-015, SUIT-016, SUIT-017
Respiratory stimulation of the FAO-pathway, F, by fatty acid FA in the presence of malate M. Malate is a type N substrate (N), required for the F-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration. Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. High concentration of malate, typically 2 mM, saturates the N-pathway.
Abbreviation: OctM
Reference: MiPNet21.06 SUIT RP, SUIT reference protocol
- More details
OctML
FAO in the LEAK state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011
-
OctMP
FAO in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007
- DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011
-
OctME
FAO in the ET state can be evaluated in the following SUIT protocols:
MitoPedia concepts:
SUIT state
