Difference between revisions of "OctM pathway control state"
Line 7: | Line 7: | ||
'''SUIT protocols:''' [[SUIT-002]], [[SUIT-015]], [[SUIT-016]], [[SUIT-017]] | '''SUIT protocols:''' [[SUIT-002]], [[SUIT-015]], [[SUIT-016]], [[SUIT-017]] | ||
{{Template:SUIT F(N)}}{{Template:SUIT M_low}} | {{Template:SUIT F(N)}} {{Template:SUIT M_low}} {{Template:SUIT M_high}} | ||
|info=[[MiPNet21.06 SUIT reference protocol]], [[SUIT reference protocol]] | |info=[[MiPNet21.06 SUIT reference protocol]], [[SUIT reference protocol]] |
Revision as of 16:52, 20 May 2019
Description
OctM: Octanoylcarnitine & Malate.
MitoPathway control state: F
SUIT protocols: SUIT-002, SUIT-015, SUIT-016, SUIT-017
Respiratory stimulation of the FAO-pathway, F, by fatty acid FA in the presence of malate M. Malate is a type N substrate (N), required for the F-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration. Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. High concentration of malate, typically 2 mM, saturates the N-pathway.
Abbreviation: OctM
Reference: MiPNet21.06 SUIT reference protocol, SUIT reference protocol
MitoPedia concepts:
SUIT state
- More details
OctML
FAO in the LEAK state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011
-
OctMP
FAO in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007
- DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011
-
OctME
FAO in the ET state can be evaluated in the following SUIT protocols: