Mitroshina 2017 Mod Technol Med
Mitroshina ЕV, Abogessimengane BZ, Urazov МD, Hamraoui I, Mishchenko ТА, Astrakhanova ТА, Shchelchkova NА, Lapshin RD, Shishkina ТV, Belousova II, Mukhina IV, Vedunova МV (2017) Adaptive role of glial cell line-derived neurotrophic factor in cerebral ischemia. Mod Technol Med 9:68-76. |
Mitroshina ЕV, Abogessimengane BZ, Urazov МD, Hamraoui I, Mishchenko ТА, Astrakhanova ТА, Shchelchkova NА, Lapshin RD, Shishkina ТV, Belousova II, Mukhina IV, Vedunova МV (2017) Mod Technol Med
Abstract: The aim of the investigation was to study the effect of glial cell line-derived neurotrophic factor (GDNF) on animals` resistance to cerebral-ischemia-induced damage.
Materials and Methods. In vivo studies were carried out on C3H male mice weighing 18–40 g. Ischemia modeling was performed by bilateral irreversible occlusion of both carotid arteries. A neurological status as well as an orientative-exploratory behavior of experimental animals and their learning capability in the post-ischemic period were analyzed by using “Open field” and “Passive avoidance” tests. In addition, high-resolution respirometer Oxygraph-2k (Oroboros, Austria) was applied to study an oxygen uptake rate of brain mitochondria in ischemic conditions.
Results. GDNF application in bilateral occlusion of carotid arteries was found to contribute to the neurological status recovery. Moreover, it normalizes oxygen uptake rate of mitochondria in the post-ischemic period.
Conclusion. GDNF has a marked neuroprotective and antihypoxic effect under ischemia modeling in vivo. • Keywords: Glial cell line-derived neurotrophic factor, GDNF, Neuroprotection, Cerebral ischemia • Bioblast editor: Kandolf G • O2k-Network Lab: RU Nizhny Novgorod Mukhina I
Labels: MiParea: Respiration, Pharmacology;toxicology
Stress:Ischemia-reperfusion Organism: Mouse Tissue;cell: Nervous system Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS
Pathway: N, S
HRR: Oxygraph-2k
2017-07