MitoKit-CII/Succinate-nv: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Snv | |abbr=Snv | ||
|description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[succinate]] in the intracellular space. | |description='''MitoKit-CII/Succinate-nv''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing [[succinate]] in the intracellular space. Abliva #: 01-118-s4 | ||
|info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | |info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | ||
}} | }} | ||
__TOC__ | __TOC__ | ||
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::::# Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO. | ::::# Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO. | ||
::::# Divide into 50 µL portions. | ::::# Divide into 50 µL portions. | ||
::::# Store up to | ::::# Store up to 4 months at -20 °C or up to 1 week at room temperature. | ||
:::» '''O2k manual titrations''' | :::» '''O2k manual titrations''' | ||
Revision as of 09:57, 7 December 2020
- high-resolution terminology - matching measurements at high-resolution
MitoKit-CII/Succinate-nv
Description
MitoKit-CII/Succinate-nv (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space. Abliva #: 01-118-s4
Abbreviation: Snv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
Application in HRR
- Succinate-nv: 25 mg, MW=262.2 g.mol-1
- Preparation of 1 M stock solution:
- Dissolve 25 mg Snv in 95.3 µL DMSO.
- Preparation of 1 M stock solution:
- Succinate-nv: 25 mg, MW=262.2 g.mol-1
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO.
- Divide into 50 µL portions.
- Store up to 4 months at -20 °C or up to 1 week at room temperature.
- Preparation of 100 mM stock solution of Snv (from the 1 M stock):
- » O2k manual titrations
- Event name: Snv
- Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 50 µM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- » O2k manual titrations
MitoPedia topics: Media for respirometry
