MiPNet23.01 IOC126 Innsbruck AT: Difference between revisions

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Revision as of 12:20, 12 December 2017

                

MiPNet23.01 IOC126 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126.


Oroboros (2018-01-24) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


General information

The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (TPP+/O2k-TPP+ ISE-Module) and amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) Furthermore, data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
data analysis


Venue

Information on travel and venue:IOC Innsbruck.
» Hotel suggestion: Hotel Weisses Kreuz
Mention "Oroboros" when booking in order to receive a discount.

Preliminary programme

Wednesday 24th Topic Room
09:00 Get-together: Introduction of participants and their research interests MiPArt
09:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. See: Abstract. MiPArt
10:00 O2k-MultiSensor overview, DatLab 7, DatLab 7 Protocols MiPArt
10:30 Coffee/Tea
11:00 Determination of mitochondrial membrane potential (mtMP) with potentiometric TPP+/O2k-TPP+ ISE-Module - Introduction MiPArt
11:45 Lunch break
13:15 Hands-on: Assembly and maintenance of TPP and reference electrode MiPNet15.03 O2k-MultiSensor-ISE MiPArt
14:30 Hands-on: Set up of the instruments with TPP and reference electrodes + TPP calibration MiPNet15.03 O2k-MultiSensor-ISE MitoFit ORO Lab
16:30 Coffee/Tea
17:00 Hands-on continued: TPP chemical background MiPNet14.05 TPP-mtMembranePotential MiPArt
Thursday 25th Topic Room
09:00 Introduction in planning and performing a TPP experiment; instrumental background oxygen flux in the presence of the TPP electrode O2k-background MiPNet14.06 Instrumental O2 background MiPArt
09:30 Hands-on: Calibration of MiPNet15.08 TPP electrode MitoFit ORO Lab
10:30 Coffee/Tea
11:00 Hands-on continued TPP: experiment with mouse brain mitochondria MitoFit ORO Lab
12:30 Lunch break
14:00 From the TPP signal to mt-membrane potential - Guide through the Excel templates. MiPNet14.05 TPP-mtMembranePotential mathematics MiPArt
14:30 Analysis of TPP experiment - discussion on mitochondrial membrane potential, MiPNet14.05 TPP-mtMembranePotential mathematics MiPArt
16:00 Coffee/Tea
16:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab
17:00 Determination of mitochondrial membrane potential (mtMP) with amperometric method (TMRM, safranin/O2k-Fluo LED2-Module) - introduction MitoFit ORO Lab
17:30 Hands-on: Set up of the instruments with LED2-Module, MiPNet17.05 O2k-Fluo LED2-Module,

Calibration of Safranin/TMRM, chemical background experiment

MitoFit ORO Lab
Friday 26th Topic Room
09:00 Introduction in planning and performing an experiment with Safranin/TMRM MiPArt
09:30 Hands-on: O2k setup for air calibration MitoFit ORO Lab
10:30 Coffee/Tea
11:00 Hands-on: Calibration of Safranin/TMRM, experiment with mouse brain mitochondria/HEK cells MitoFit ORO Lab
12:30 Lunch break
14:00 Analysis of TMRM/Safranin experiment - discussion MiPArt
15:00 Coffee/Tea
16:30 Round table: questions MiPArt
17:00 Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications, the Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions MiPArt
18:00 Farewell activity



Registration

Registration form

Tutors and Lecturers

Tutors

Lecturers and assistance

List of participants

Preliminary list
Participant Institution
Kandel Sunil Mani US WI Milwaukee Dash RK - Medical College of Wisconsin
Smirnova Irina SE Stockholm Smirnova I - Stockholm University
Magdalena Labieniec-Watala Labieniec-Watala Magdalena PL Lodz Watala C - University of Lodz
Janssen Connie NL Leiden Lindeman JH - Leiden University Medical Center
de Kok Michéle NL Leiden Lindeman JH - Leiden University Medical Center
Steffen Anja NL Leiden Lindeman JH - Leiden University Medical Center
Siewiera Karolina Siewiera Karolina PL Lodz Watala C - University of Lodz
Michalak Slawomir Michalak Slawomir PL Poznan Michalak S - Poznan University of Medical Sciences
Fernandez-Vizarra Erika UK Cambridge Zeviani M - University of Cambridge


Contact

[email protected]
Oroboros Instruments
High-Resolution FluoRespirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png


Labels:






ORO, IOC, MitoFit, Next, 2018 


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