MiPNet18.06 Amplex-Yeast: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/f/f9/MiPNet18.06_Amplex-Yeast.pdf |Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/f/f9/MiPNet18.06_Amplex-Yeast.pdf |Bioblast pdf]] | ||
|authors= | |authors=Oroboros | ||
|year=2015-06-15 | |year=2015-06-15 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
| Line 9: | Line 9: | ||
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2). | Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2). | ||
:>> Product: [ | :>> Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]] | ||
|mipnetlab=AT Innsbruck | |mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Revision as of 13:14, 13 July 2017
MiPNet18.06 Amplex-Yeast
 |
» 
Oroboros (2015-06-15) Mitochondr Physiol Network
Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4 »Versions
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the O2k-Fluorescence LED2-Module. Extracellular H2O2-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H2O2 production (Fig. 2).
- >> Product: Oroboros O2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Saccharomyces cerevisiae, Fungi
Preparation: Intact cells
Regulation: Oxygen kinetics, Uncoupler Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor
See also
- A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - »Bioblast link«
