MiPNet18.06 Amplex-Yeast

                

---

---

MiPNet18.06 Amplex-Yeast

O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.

»

Oroboros (2015-06-15) Mitochondr Physiol Network

Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4 »Versions

Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the O2k-Fluorescence LED2-Module. Extracellular H₂O₂-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H₂O₂ production (Fig. 2).

>> Product: Oroboros O2k, O2k-Catalogue

• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom

Labels: MiParea: Respiration 

Organism: Saccharomyces cerevisiae, Fungi 

Preparation: Intact cells 

Regulation: Oxygen kinetics, Uncoupler  Coupling state: ROUTINE, ET 

HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol 

O2k-Demo, O2k-MultiSensor 

See also

• A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - »Bioblast link«