MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver
Eigentler A, Fontana-Ayoub M, Gnaiger E (2012) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15. |
Β» MiPNet17.15. Open Access, PBI-Shredder HRR-Set
OROBOROS (2012) Mitochondr Physiol Network
Abstract: A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR. β’ Keywords: Tissue homogenate, Shredder
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Manual, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
mt-Preparation
- Version 1: 2012
- Version 3: 2013-01-15