MiPNet17.04 CitrateSynthase: Difference between revisions
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{{Publication | {{Publication | ||
|title=Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr. Physiol. Network 08.14. | |title=Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr. Physiol. Network 08.14. | ||
|info=[http://www.oroboros.at/index.php?id=protocols_cs MiPNet08.14] | |||
|authors=Kuznetsov AV, Lassnig B, Gnaiger E | |authors=Kuznetsov AV, Lassnig B, Gnaiger E | ||
|year=* | |year=* | ||
|journal=Mitochondr. Physiol. Network | |journal=Mitochondr. Physiol. Network | ||
|abstract=Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al., 1970; Willimas et al., 1986; Hood et al., 1989), although this role of CS has been questioned in developmental (Drahota et al., 2004) and age-related studies (Marin-Garcia et al., 1998). Proliferation of mitochondria in pathological states is normally associated with an increase in citrate synthase activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein. Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications (Kuznetsov et al., 2002; Renner et al., 2003; Hütter el al., 2004). | |abstract=Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al., 1970; Willimas et al., 1986; Hood et al., 1989), although this role of CS has been questioned in developmental (Drahota et al., 2004) and age-related studies (Marin-Garcia et al., 1998). Proliferation of mitochondria in pathological states is normally associated with an increase in citrate synthase activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein. Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications (Kuznetsov et al., 2002; Renner et al., 2003; Hütter el al., 2004). | ||
|keywords=Enzymes | |keywords=Enzymes | ||
| | |mipnetlab=AT_Innsbruck_OROBOROS | ||
|discipline=Mitochondrial Physiology | |||
|articletype=Protocol; Manual, MiPNet-online Publication | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |instruments=Oxygraph-2k, Protocol, MiPNet-Publication | ||
|preparations=Enzyme | |preparations=Enzyme | ||
|topics=Mitochondrial Biogenesis; Mitochondrial Density | |topics=Mitochondrial Biogenesis; Mitochondrial Density | ||
| | |discipline=Mitochondrial Physiology | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
Revision as of 17:12, 13 May 2011
| Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr. Physiol. Network 08.14. |
Kuznetsov AV, Lassnig B, Gnaiger E (
) Mitochondr. Physiol. Network
Abstract: Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al., 1970; Willimas et al., 1986; Hood et al., 1989), although this role of CS has been questioned in developmental (Drahota et al., 2004) and age-related studies (Marin-Garcia et al., 1998). Proliferation of mitochondria in pathological states is normally associated with an increase in citrate synthase activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein. Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications (Kuznetsov et al., 2002; Renner et al., 2003; Hütter el al., 2004). • Keywords: Enzymes
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Preparation: Enzyme
Regulation: Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
