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| |year=2018-11-18 | | |year=2018-11-18 |
| |journal=Mitochondr Physiol Network | | |journal=Mitochondr Physiol Network |
| |abstract= Oroboros (2018) MitoPathways at the Q-junction: mouse skeletal muscle fibers. Mitochondr Physiol Network 12.01(03): Suppl T-issue. ยป [http://www.bioblast.at/index.php/File:MiPNet12.01 Versions] | | |abstract= Oroboros (2018) MitoPathways at the Q-junction: mouse skeletal muscle fibers. Mitochondr Physiol Network 12.01(03): Suppl T-issue. |
| {{MiPNet pdf page linking to MitoPedia}} | | {{MiPNet pdf page linking to MitoPedia}} |
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| |additional=MitoPathways, O2k-Demo, O2k-Core | | |additional=MitoPathways, O2k-Demo, O2k-Core |
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| == Limitations of the SUIT protocol ==
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| === Maximum OXPHOS and ET-capacity ===
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| :::: Evaluation of maximum respiratory capacities requires titration of further substrates activating additional [[respiratory complexes]] at the Q-junction ([[Electron-transferring flavoprotein complex |CETF]] and [[glycerophosphate dehydrogenase complex |CGpDH<]]).
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| === Malate concentration ===
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| :::: The [[malate]] concentration was 2 mM, to saturate N-linked respiration. However, at 2 mM malate, the fumarate concentration is increased to a level which inhibits succinate dehydrogenase. Then NS- and S-linked respiratory capacities are underestimated. A malate concentration of 0.5 mM, which saturates N-linked respiration and inhibits S-linked respiration to a lesser extent, represents and improved standard.
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| ::::ยป [[Talk:Malate |Optimum malate concentration in SUIT protocols]]
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| === ROX correction ===
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| :::: The fact that ROX was higher in the NS-substrate state compared to N-linked LEAK respiration indicates that ROX is partially controlled by the substrate state. Therefore, a single measurement of ROX cannot be applied for correction of total oxygen consumption in the different substrate states. Total respiration, therefore, represents apparent coupling states ''L''ยด, ''P''ยด and ''E''ยด (Fig. 1). ROX correction is possible in the present experiment only for NS- and S-linked respiration. [[Azide]] inhibits not only CIV but other heme-based oxidases and peroxidases, and therefore may interfere with ROX beyond blocking respiratory electron transfer. Based on this argument, a combination of CII- and CIII-inhibitors (malonic acid, antimycin A, myxothiazol) may yield more consistent results, although any ROS scavenged by cytochrome ''c'' may in the absence of a CIV-inhibitor result in respiratory oxygen consumption through CIV.
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Latest revision as of 12:30, 4 December 2019
MiPNet12.01 Suppl T-issue
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
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ยป ยปVersions
Oroboros (2018-11-18) Mitochondr Physiol Network
Abstract: Oroboros (2018) MitoPathways at the Q-junction: mouse skeletal muscle fibers. Mitochondr Physiol Network 12.01(03): Suppl T-issue.
- Open the pdf document above.
โข O2k-Network Lab: AT_Innsbruck_Oroboros
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, ROUTINE, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k, O2k-Protocol
MitoPathways, O2k-Demo, O2k-Core
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