Kimball 2016 Thesis: Difference between revisions
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{{Publication | {{Publication | ||
|title=Kimball RL (2016) The role of hypoxia on pyruvate kinase M2, mammalian target of rapamycin, mitochondrial function, and cell invasion in the trophoblast. Master Thesis p55. | |title=Kimball RL (2016) The role of hypoxia on pyruvate kinase M2, mammalian target of rapamycin, mitochondrial function, and cell invasion in the trophoblast. Master Thesis p55. | ||
|info=[http://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=6722&context=etd | |info=[http://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=6722&context=etd Open access] | ||
|authors=Kimball RL | |authors=Kimball RL | ||
|year=2016 | |year=2016 | ||
|journal= | |journal=Thesis Brigham Young University | ||
|abstract=Intrauterine growth restriction (IUGR) is a disease that affects many pregnant women and their growing fetuses. Hypoxia and shallow invasion are key characteristics of IUGR. A lack of oxygen has been shown to affect the mitochondria, which produces the energy in the cell. Pyruvate Kinase M2 (PKM2) is a metabolic enzyme known to be involved during hypoxia. Our objective was to determine the role of hypoxia in mitochondrial function, trophoblast cell | |abstract=Intrauterine growth restriction (IUGR) is a disease that affects many pregnant women and their growing fetuses. Hypoxia and shallow invasion are key characteristics of IUGR. A lack of oxygen has been shown to affect the mitochondria, which produces the energy in the cell. Pyruvate Kinase M2 (PKM2) is a metabolic enzyme known to be involved during hypoxia. Our objective was to determine the role of hypoxia in mitochondrial function, trophoblast cell | ||
invasion, and PKM2. | invasion, and PKM2. | ||
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Hypoxic treated cells showed a decrease in mitochondrial function, invasion, and nuclear and cytostolic PKM2. Shikonin treated cells showed a decrease in invasion and nuclear PKM2, and an increase in mitochondrial function. | Hypoxic treated cells showed a decrease in mitochondrial function, invasion, and nuclear and cytostolic PKM2. Shikonin treated cells showed a decrease in invasion and nuclear PKM2, and an increase in mitochondrial function. | ||
|keywords=Human cytotrophoblast Sw71 cells | |||
|mipnetlab=US UT Provo Bikman BT | |mipnetlab=US UT Provo Bikman BT | ||
}} | }} | ||
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|organism=Human | |organism=Human | ||
|tissues=Genital | |tissues=Genital | ||
|model cell lines=Other cell lines | |||
|preparations=Permeabilized cells | |preparations=Permeabilized cells | ||
|injuries=Oxidative stress;RONS | |injuries=Oxidative stress;RONS | ||
|diseases=Other | |diseases=Other | ||
|couplingstates=OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, CI&II, ROX | |substratestates=CI, CII, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2016-06 | ||
}} | }} | ||
Revision as of 13:27, 8 September 2016
| Kimball RL (2016) The role of hypoxia on pyruvate kinase M2, mammalian target of rapamycin, mitochondrial function, and cell invasion in the trophoblast. Master Thesis p55. |
Kimball RL (2016) Thesis Brigham Young University
Abstract: Intrauterine growth restriction (IUGR) is a disease that affects many pregnant women and their growing fetuses. Hypoxia and shallow invasion are key characteristics of IUGR. A lack of oxygen has been shown to affect the mitochondria, which produces the energy in the cell. Pyruvate Kinase M2 (PKM2) is a metabolic enzyme known to be involved during hypoxia. Our objective was to determine the role of hypoxia in mitochondrial function, trophoblast cell invasion, and PKM2.
Human placenta tissues were collected at term to characterize the mitochondria in the cells. Human trophoblast cells were used to determine PKM2 expression. Treatment was with hypoxia, Shikonin, or Rapamycin to observe mitochondrial function and invasion characteristics of the trophoblast cells. The cells were lysed after treatment and western blots were performed to view PKM2 expression.
Hypoxic treated cells showed a decrease in mitochondrial function, invasion, and nuclear and cytostolic PKM2. Shikonin treated cells showed a decrease in invasion and nuclear PKM2, and an increase in mitochondrial function. • Keywords: Human cytotrophoblast Sw71 cells
• O2k-Network Lab: US UT Provo Bikman BT
Labels: MiParea: Respiration
Pathology: Other
Stress:Oxidative stress;RONS
Organism: Human
Tissue;cell: Genital
Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
2016-06
