Friederich-Persson 2013 Abstract MiP2013

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*Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013) Potassium controls rat mitochondrial function: In vivo* and in vitro considerations. Mitochondr Physiol Network 18.08.**

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MiP2013, Book of Abstracts Open Access

Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013)

Event: MiPNet18.08_MiP2013

The intracellular potassium concentration, [K+], varies depending on tissue type and is about 60 mM in kidney proximal tubular cells whereas it is approximately 120 mM in cardiac myocytes. Intracellular [K+] may therefore affect mitochondria function.

By measuring the respiratory function and swelling of mitochondria isolated from kidney cortex and heart at [K+] ranging from 15 mM to 146 mM we showed that [K+] controls mitochondria function in the heart. Increasing [K+] resulted in increased resting state respiration and decreased respiratory control ratio (RCR) in kidney cortex mitochondria. The reduced RCR corresponded with mitochondria swelling, indicating failing mitochondria. The effects of K+ in kidney mitochondria was prevented by inhibitors of ATP-sensitive K+-channels and voltage-gated K+-channels (glibenclamide and by 4-aminopyridine, respectively). On the contrary, RCR in heart mitochondria increased with increasing [K+] and was unaffected by K+channel inhibitors.

These results demonstrate [K+] control of mitochondria function in rat kidney and heart.

• O2k-Network Lab: SE Uppsala Liss P

Labels: MiParea: Respiration, mt-Structure;fission;fusion

Organism: Rat Tissue;cell: Heart, Kidney Preparation: Isolated mitochondria

Regulation: Flux control, Ion;substrate transport

HRR: Oxygraph-2k

MiP2013, S06

Affiliations and author contributions

1 - Dept of Medical Cell Biology, Uppsala University, Sweden

2 - Dept of Medicine, Division of Hypertension and Nephrology, Georgetown University Medical Center, Washington DC, USA;

3 - Dept of Medical and Health Sciences, Linköping University, Sweden;

4 - Center for Medical Image Science and Visualization, Linköping University, Sweden.

Email: [email protected]