Cytochrome c: Difference between revisions
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|type=Substrate ETS | |type=Substrate ETS | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
''' ''c'': Cytochrome ''c'' ''' ( | ''' ''c'': Cytochrome ''c'' ''' (Sigma C 7752, 50mg, store at -20 Β°C): FW = 12500) | ||
Β | |||
'''Preparation of 4 mM Cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O): | |||
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening! | |||
::1) weigh | ::1) weigh 50 mg of Cytochrome ''c'' into a small glass vial. Difficult to weigh, since the powder appears to be electrostatic. | ||
::2) add | ::2) add 1 ml H<sub>2</sub>O. Dissolves easily. | ||
::3) | ::3) divide into 0.2 ml portions Β | ||
::4) store frozen at -20 Β°C | |||
:: | |||
'''Oxygraph-2k Manual Titrations:'''Β [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:'''Β [[MiPNet09.12]] | ||
::* Titration volume: | ::* Titration volume: 5 Β΅l using a 25 Β΅l syringe | ||
::* Final conc. in 2 ml O2k-chamber: 10 | ::* Final conc. in 2 ml O2k-chamber: 10 Β΅M |
Revision as of 11:34, 22 November 2010
Description
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
Labels:
Application in HRR
c: Cytochrome c (Sigma C 7752, 50mg, store at -20 Β°C): FW = 12500)
Preparation of 4 mM Cytochrome c solution (dissolved in H2O):
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!
- 1) weigh 50 mg of Cytochrome c into a small glass vial. Difficult to weigh, since the powder appears to be electrostatic.
- 2) add 1 ml H2O. Dissolves easily.
- 3) divide into 0.2 ml portions
- 4) store frozen at -20 Β°C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 Β΅l using a 25 Β΅l syringe
- Final conc. in 2 ml O2k-chamber: 10 Β΅M