From Bioblast
Description
Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.
Abbreviation: CS
Reference: MiPNet17.04 CitrateSynthase
Normalization of respiratory flux for CS as a mitochondrial marker
Communicated by Gnaiger Erich last update 2020-04-19
- Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, JO2/CS [pmolβsΒ-1βIUΒ-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
- Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmolβs-Β1βmLΒ-1βIUΒ-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2].
- Gnaiger Erich et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - Β»Bioblast linkΒ«
- Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - Β»Bioblast linkΒ«
MitoPedia topics:
Enzyme