Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | |title=Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | ||
|info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum] | |info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum] | ||
|authors=Chavanelle V | |authors=Chavanelle V | ||
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|diseases=Diabetes | |diseases=Diabetes | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
| | |pathways=F, N | ||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine | ||
}} | }} |
Latest revision as of 15:57, 7 November 2016
Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Chavanelle V (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
Fatty oxidation in permeabilised db/db mice muscle fibres, in Mir05 + Cr +37ยฐC. We found that ADP-stimulated respiration in presence of 0,02 mM of palmitoyl-carnitine (OXPHOS) is very close to the non-ADP-stimulated respiration (LEAK state) when working with these conditions. (Values around 14 to 20 (pmol/s/mg w.t.) for LEAK state and 15 to 24 (pmol/s/mg w.t.) for fatty acid triggered-OXPHOS). These data suggest either a very poor ability for this animal model to use palmitoylcarnitine or a very high LEAK state (or both!)
Protocol
- 2,2 mL mir05+Cr was added in the chambers and oxygen was added to the medium up to 400 โ 450 nmol/mL
- Chambers were closed
- 10ยตM Amplex Red was added to the chambers
- 2 U HRP was added
- 10 U SOD was added
- 20ยตM Palmitoyl-Carnitine was added
- 2 mM Malate was added
- 1 to 3 mg wet permeabilised muscle fibers was added
- LEAK state was recorded
- Mg+ was added
- 2,5 mM ADP was added
- Pal-C stimulated OXPHOS was recorded
The tissue used was skeletal muscle (Gastrocnemius)
โข Keywords: Amplex Red
Labels: Pathology: Diabetes
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS
Pathway: F, N
Palmitoylcarnitine