Ap5A: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 15:18, 30 November 2020
- high-resolution terminology - matching measurements at high-resolution
Ap5A
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).
Application in HRR
- Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt Sigma D4022, store at -20 °C; molecular weight: 1026.28
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O):
- For a 2 mg glass vial: dissolve in 19.49 µL H2O
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 50 µM.
References
Abbreviation: Ap5A
MitoPedia topics:
Inhibitor
