Ap5A: Difference between revisions
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|abbr=Ap5A | |abbr=Ap5A | ||
|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ 2 ADP). | |description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ 2 ADP). | ||
}} | |||
__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Ap5A: P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 ยฐC; molecular weight: 1026.28 | {{Chemical_description | ||
|abbr=Ap5A | |||
|trivial name=Ap5A | |||
|complete name=P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt | |||
|chem formula=C<sub>2</sub>0H<sub>29</sub>N<sub>10</sub>O<sub>22</sub>P<sub>5</sub> ยท 5 Na | |||
|molar mass=1026.28 | |||
|vendor=Sigma-Aldrich | |||
|product number=D4022 | |||
|store at=-20 ยฐC | |||
|sensitivity= | |||
|cas=4097-04-5 | |||
|h statements= | |||
|h info= | |||
}}<!--:::: '''Ap5A: P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 ยฐC; molecular weight: 1026.28 | |||
::::ย <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>--> | |||
:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O) | |||
ย | ::::# Dissolve content of Sigma 2 mg glass vial in 19.49 ยตL H<sub>2</sub>O | ||
:::: | |||
ย | |||
::::# Store at -20 ยฐC. | ::::# Store at -20 ยฐC. | ||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | :::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 1 ยตL using a 10 ยตL syringe | ::::* Titration volume ('''2-mL O2k-chamber'''): 1 ยตL using a 10 ยตL Hamilton syringe. | ||
::::* Final concentration: 50 ยตM. | ::::* Final concentration: 50 ยตM. | ||
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::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ||
}} | ย | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} |
Latest revision as of 10:25, 21 June 2021
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ 2 ADP).
Abbreviation: Ap5A
Application in HRR
- Ap5A: Ap5A (P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt; C20H29N10O22P5 ยท 5 Na), Sigma-Aldrich: D4022, store at -20 ยฐC, CAS: 4097-04-5, M = 1026.28 gยทmol-1
- Preparation of 100 mM stock solution (dissolved in H2O)
- Dissolve content of Sigma 2 mg glass vial in 19.49 ยตL H2O
- Store at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 50 ยตM.
References
MitoPedia topics: Inhibitor