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TissueModels
TissueModels - Establishing tissue-specific cell models with culture medium additives
TissueModels: FFG BRIDGE Young Scientists
- BRIDGE Young Scientists 2. Call - Project No. 878654, Medical University Innsbruck, Institute of Human Genetics
- Duration: 36 months
- Start: 2020-04-01
About TissueModels
- Led by the Institute of Human Genetics at the Medical University Innsbruck, the TissueModels project aims to establish physiological models for the specific tissue type under investigation by defining medium compositions that preserve or re-establish structural and metabolic characteristics of cells during in vitro culture.
Aims
- The specific aims are:
- To identify medium composition with emphasis on the levels of selected fatty acids and of glucose that support growth and viability of cell lines originally derived from different tissues.
- To evaluate the metabolic consequences of incubation with selected media with emphasis on mitochondrial energetics.
- To assess the membrane lipid architecture of cells exposed to selected media to evaluate to which extent the remodeling and/or de novo synthesis of cell lipids were altered and to compare the lipidome with that of the original tissue.
- To refine prototypes (exact growth conditions and media additives) that allow to obtain tissue specific lipid patterns and energetic fingerprints in cultured cells.
- The specific aims are:
Coordinator
- Dr. Markus A. Keller - Dr. Markus A. Keller, Institute of Human Genetics, Medical University Innsbruck- https://www.i-med.ac.at/imcbc/staff_doc/Keller_Markus.html
Network
Participants
- Institute of Human Genetics, Medical University Innsbruck
- Dr. Markus A. Keller - Project Coordinator, WP 1 & 5 Leader
- Marie-Luise Edenhofer - WP 2 Leader
- Institute of Human Genetics, Medical University Innsbruck
- Jakob Koch - WP 4 Leader
- Oroboros Instruments GmbH - www.oroboros.at
- Erich Gnaiger, Ph.D. - PI
- Luiza Cardoso, Ph.D. - WP 3 Leader
- Verena Laner - Oroboros project management
- Lisa Tindle-Solomon - Oroboros project management
- Oroboros Instruments GmbH - www.oroboros.at
Oroboros project involvement
- Oroboros Instruments will provide both infrastructure and the expertise required to conduct the respirometric measurements of the project.
MitoKIN Events
When | Where | |
---|---|---|
Oemer 2021 J Lipid Res | 2021 | Oemer G, Edenhofer ML, Wohlfarter Y, Lackner K, Leman G, Koch J, Cardoso LHD, Lindner HH, Gnaiger E, Dubrac S, Zschocke J, Keller MA (2021) Fatty acyl availability modulates cardiolipin composition and alters mitochondrial function in HeLa cells. https://doi.org/10.1016/j.jlr.2021.100111 |
Links
- Coming soon
Support
This project has received funding from the Austrian Reserach Promotion Funding Agency (FFG).