Legkun 2014 Abstract IOC 2014-04 Schroecken: Difference between revisions
(Investigation on enzyme activity ratio under "shunt" respiration) |
(Investigation on alternative electron transport in respiratory chain) |
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{{Abstract | {{Abstract | ||
|title=Legkun (2014) | |title=Legkun (2014) Investigation on alternative electron transport in respiratory chain. Mitochondr Physiol Network 19.02. | ||
|authors=Legkun G, Motovilov K | |authors=Legkun G, Motovilov K | ||
|year=2014 | |year=2014 | ||
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|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|enzymes=Complex I | |enzymes=Complex I | ||
|topics=ADP, Inhibitor, Uncoupler | |||
|instruments=Oxygraph-2k, TPP | |instruments=Oxygraph-2k, TPP | ||
|link= | |link= | ||
|mammals_and_models= | |mammals_and_models= | ||
}} | }} |
Revision as of 15:52, 10 March 2014
Legkun (2014) Investigation on alternative electron transport in respiratory chain. Mitochondr Physiol Network 19.02. |
Link:
Legkun G, Motovilov K (2014)
Event: IOC 2014-04 Schroecken
Electron Transport Chain might function in many different states including physiological conditions and cannot be considered as united consecutive system. Major side mechanism of electron transport is shunt, set by hydrophilic quinones(such as duroquinine and menadione(Vitamin K3)) which oxidize NADH and bring electrons to different sites of respiratory chain, establishing thereby alternative way for electron transport. Foresaid way is performed by DT-diaphorase, an alternative two-electron quinone reductase that βexcludesβ Complex I from ETC. Activity rate ratio of respiratory enzymes under such conditions was investigated on preparation of rat liver mitochondria. It is important to notice that limiting stage of ETC under normal conditions is terminal stage β proton transfer through membrane surface β water boundary. However, under uncoupled condition another steps linked to individual enzyme function become limiting stage. To obtain insight into dependence βshuntβ respiration on transmembrane potential, we investigate substrate oxidation rate depending on uncouplers of different structure (PCP, FCCP et al.) simultaneously with membrane potential of isolated mitochondria.
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Liver
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Enzyme: Complex I
Regulation: ADP, Inhibitor, Uncoupler
HRR: Oxygraph-2k, TPP