Kuznetsov 2008 Nat Protoc: Difference between revisions
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==Corrections required== | ==Corrections required== | ||
* Table 1: ''Respiration of rat liver homogenate, 30 Β°C'' is actually mechanically permeabilized pig liver measured at 37 Β°C ([[Kuznetsov_2002_Analyt Biochem]]). | |||
* The protocol (Nat Protoc) for permeabilized fibres lacks consideration of oxygen limitation encountered when incubations are performed at or below air saturation ([[Kuznetsov_1998_BTK]]; [[Gnaiger_2003_Adv Exp Med Biol]]). For a discussion, see MitoPedia: [[Permeabilized muscle fibres]]. | |||
* Protocols (Nat Protoc) are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations ([[Gnaiger 2007 MitoPathways|Gnaiger 2007]]; [[Pesta 2012 Methods Mol Biol]]). |
Revision as of 07:48, 23 March 2012
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat Protoc 3: 965-976. |
Kuznetsov AV, Veksler V, Gellerich FN, Saks V, Margreiter R, Kunz WS (2008) Nat. Protoc.
Abstract: Analysis of mitochondrial function is central to the study of intracellular energy metabolism, mechanisms of cell death and pathophysiology of a variety of human diseases, including myopathies, neurodegenerative diseases and cancer. However, important properties of mitochondria differ in vivo and in vitro. Here, we describe a protocol for the analysis of functional mitochondria in situ, without the isolation of organelles, in selectively permeabilized cells or muscle fibers using digitonin or saponin. A specially designed substrate/inhibitor titration approach allows the step-by-step analysis of several mitochondrial complexes. This protocol allows the detailed characterization of functional mitochondria in their normal intracellular position and assembly, preserving essential interactions with other organelles. As only a small amount of tissue is required for analysis, the protocol can be used in diagnostic settings in clinical studies. The permeabilization procedure and specific titration analysis can be completed in 2 h.
β’ O2k-Network Lab: EE_Tallinn_Saks VA, FR_Grenoble_Saks VA
Labels:
Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
Corrections required
- Table 1: Respiration of rat liver homogenate, 30 Β°C is actually mechanically permeabilized pig liver measured at 37 Β°C (Kuznetsov_2002_Analyt Biochem).
- The protocol (Nat Protoc) for permeabilized fibres lacks consideration of oxygen limitation encountered when incubations are performed at or below air saturation (Kuznetsov_1998_BTK; Gnaiger_2003_Adv Exp Med Biol). For a discussion, see MitoPedia: Permeabilized muscle fibres.
- Protocols (Nat Protoc) are restricted to simple substrate supply (separate CI- or CII-electron entry into the ETS), whereas full OXPHOS capacity can be obtained only with physiological CI+II substrate combinations (Gnaiger 2007; Pesta 2012 Methods Mol Biol).