SUIT-003 Ce1;ce1SD;ce2Omy;ce3U-: Difference between revisions
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::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama | ::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling-control protocol]] ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama | ||
[[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | [[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | ||
[[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | [[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | ||
== Coupling control protocol == | == Coupling-control protocol == | ||
::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma | ::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma | ||
Revision as of 16:24, 2 June 2020
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce1SD;ce2Omy;ce3U-
Description
Abbreviation: FNS(Oct,PGM)
Reference: B SUIT-003 Stadlmann 2006 Cell Biochem Biophys
O2k-Application: O2
- SUIT protocol pattern: complex cell coupling-control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
Coupling-control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
ce1 | ROUTINE | Living cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
ce1SD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
ce2Omy | L+SL | LEAK (L) + S (SL) | ce and dce are in the LEAK state. |
ce3U | E+SE | ET-pathway (E) + S (SE) | ce and dce are in the ET state. |
ce4Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
ce5Ama | ROXAma | ROX |
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts:
SUIT protocol,
SUIT B