Template:SUIT text D067: Difference between revisions
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[[ | The SUIT-003 O2 ce D067 is designed to study the effect of glucose (or fructose; [[Crabtree effect]]) on ROUTINE respiration and glycolysis inhibition on LEAK-respiration and proton flux in living cells. This protocol allows us to omit oligomycin, therefore the LEAK-respiration. If the aim is to analyze only the Crabtree effect and the effect of [[2-Deoxyglucose]] on proton flux and respiration via glycolysis inhibition, [[Oligomycin]] can be omitted as well. If there is an interest in studying coupling control, oligomycin should be added to promote LEAK-respiration. To obtain more information about the Crabtree effect, it is ideal to use in parallel the protocol [[SUIT-003 O2 ce D038]] (glucose addition in ET state). [[SUIT-003 O2 ce D037]] can be extended by the cell viability test and the Complex IV module. | ||
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Revision as of 10:55, 21 February 2020
The SUIT-003 O2 ce D067 is designed to study the effect of glucose (or fructose; Crabtree effect) on ROUTINE respiration and glycolysis inhibition on LEAK-respiration and proton flux in living cells. This protocol allows us to omit oligomycin, therefore the LEAK-respiration. If the aim is to analyze only the Crabtree effect and the effect of 2-Deoxyglucose on proton flux and respiration via glycolysis inhibition, Oligomycin can be omitted as well. If there is an interest in studying coupling control, oligomycin should be added to promote LEAK-respiration. To obtain more information about the Crabtree effect, it is ideal to use in parallel the protocol SUIT-003 O2 ce D038 (glucose addition in ET state). SUIT-003 O2 ce D037 can be extended by the cell viability test and the Complex IV module.
