Oxygen signal: Difference between revisions
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== Stability of the oxygen signal == | === Stability of the oxygen signal === | ||
The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber. | The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber. | ||
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: Follow the standard instructions for [[MiPNet19.18B POS-Service|oxygen sensor service]]. | : Follow the standard instructions for [[MiPNet19.18B POS-Service|oxygen sensor service]]. | ||
=== Oxygen signal does not respond == ย | === Oxygen signal does not respond === | ||
* The oxygen signal remains at or close to zero even at high oxygen levels. | * The oxygen signal remains at or close to zero even at high oxygen levels. | ||
* The signal at air saturation is abnormally low. | * The signal at air saturation is abnormally low. |
Revision as of 23:02, 6 February 2016
Description
The oxygen signal of the O2k is transmitted from the electrochemical polarographic oxygen sensor (OroboPOS) for each of the two chambers to DatLab. The primary signal is a current mAmp which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [ยตmol.dm-3 or ยตM].
Reference: MiPNet19.18D O2k-Calibration, MiPNet06.03 POS-Calibration-SOP
MitoPedia methods: Respirometry, "DatLab" is not in the list (Respirometry, Fluorometry, Spectrophotometry) of allowed values for the "MitoPedia method" property. DatLab
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Stability of the oxygen signal
The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber.
- Instability of the oxygen signal
- There is drift of the oxygen signal over short or long periods of time.
- The signal at air saturation is abnormally high for a given gain setting.
- The zero current is high (>2.5%) and may show drift.
- Confirm and locate the problem by performing a Sensor test and following Locating a problem.
- Follow the standard instructions for oxygen sensor service.
Oxygen signal does not respond
- The oxygen signal remains at or close to zero even at high oxygen levels.
- The signal at air saturation is abnormally low.
- Check the gain setting for the affected oxygen channel.
- Response 1: The gain setting was too low, and the oxygen signal increases to the expected level after increasing the gain.
- Response 2: The gain was correct: Locate the problem using the procedure described in Locating a problem and Sensor test.