Zhan 2018 Mol Cell

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Zhan N, Wang C, Chen L, Yang H, Feng J, Gong X, Ren B, Wu R, Mu J, Li Y, Liu Z, Zhou Y, Peng J, Wang K, Huang X, Xiao S, Zuo J (2018) S-Nitrosylation targets GSNO reductase for selective autophagy during hypoxia responses in plants. Mol Cell 71:142-54.

» PMID: 30008318

Zhan N, Wang C, Chen L, Yang H, Feng J*, Gong X, Ren B, Wu R, Mu J, Li Y*, Liu Z, Zhou Y, Peng J, Wang K, Huang X, Xiao S, Zuo J (2018) Mol Cell

Abstract: Nitric oxide (NO) regulates diverse cellular signaling through S-nitrosylation of specific Cys residues of target proteins. The intracellular level of S-nitrosoglutathione (GSNO), a major bioactive NO species, is regulated by GSNO reductase (GSNOR), a highly conserved master regulator of NO signaling. However, little is known about how the activity of GSNOR is regulated. Here, we show that S-nitrosylation induces selective autophagy of Arabidopsis GSNOR1 during hypoxia responses. S-nitrosylation of GSNOR1 at Cys-10 induces conformational changes, exposing its AUTOPHAGY-RELATED8 (ATG8)-interacting motif (AIM) accessible by autophagy machinery. Upon binding by ATG8, GSNOR1 is recruited into the autophagosome and degraded in an AIM-dependent manner. Physiologically, the S-nitrosylation-induced selective autophagy of GSNOR1 is relevant to hypoxia responses. Our discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy.

Keywords: GSNO reductase, S-nitrosylation, Hypoxia responses, Nitric oxide, Selective autophagy Bioblast editor: Plangger M, Kandolf G


Labels: MiParea: Respiration 

Stress:Hypoxia  Organism: Plants 

Preparation: Intact organism 



HRR: Oxygraph-2k 

Labels, 2018-08