Wu 2018 Adv Sci

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Wu S, Zhang A, Li S, Chatterjee S, Qi R, Segura-Ibarra V, Ferrari M, Gupte A, Blanco E, Hamilton DJ (2018) Polymer functionalization of isolated mitochondria for cellular transplantation and metabolic phenotype alteration. Adv Sci 1700530.

» Open Access

Wu S, Zhang A, Li S, Chatterjee S, Qi R, Segura-Ibarra V, Ferrari M, Gupte A, Blanco E, Hamilton DJ (2018) Adv Sci

Abstract: Aberrant mitochondrial energy transfer underlies prevalent chronic health conditions, including cancer, cardiovascular, and neurodegenerative diseases. Mitochondrial transplantation represents an innovative strategy aimed at restoring favorable metabolic phenotypes in cells with dysfunctional energy metabolism. While promising, significant barriers to in vivo translation of this approach abound, including limited cellular uptake and recognition of mitochondria as foreign. The objective is to functionalize isolated mitochondria with a biocompatible polymer to enhance cellular transplantation and eventual in vivo applications. Herein, it is demonstrated that grafting of a polymer conjugate composed of dextran with triphenylphosphonium onto isolated mitochondria protects the organelles and facilitates cellular internalization compared with uncoated mitochondria. Importantly, mitochondrial transplantation into cancer and cardiovascular cells has profound effects on respiration, mediating a shift toward improved oxidative phosphorylation, and reduced glycolysis. These findings represent the first demonstration of polymer functionalization of isolated mitochondria, highlighting a viable strategy for enabling clinical applications of mitochondrial transplantation.


Bioblast editor: Kandolf G O2k-Network Lab: US LA Baton Rouge Hand SC


Labels: MiParea: Respiration, mt-Medicine  Pathology: Cancer 

Organism: Mouse  Tissue;cell: Liver  Preparation: Isolated mitochondria 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, ROX  HRR: Oxygraph-2k 

Labels, 2018-02