Vieira 2013 FASEB J
| Vieira AK, de Mattos AM, Neves FA, Bernardo AF, Cortez EC, de Souza LR, Miranda GL, de Melo Soares dos Santos V, de Souza Rodrigues-Cunha AC, Garcia-Souza EP, Sichieri R, Sanchez Moura A (2013) Hemodynamic and bioenergetics of hearts from obese adult mice overfed during early life. FASEB J 27:1130.8. |
Vieira AK, de Mattos AM, Neves FA, Bernardo AF, Cortez EC, de Souza LR, Miranda GL, de Melo Soares dos Santos V, de Souza Rodrigues-Cunha AC, Garcia-Souza EP, Sichieri R, Sanchez Moura A (2013) FASEB J
Abstract: In this study we examined heart hemodynamic and metabolism of adult obese mice overfed during lactation. Heart left ventricle from obese (OG) and control (CG) groups were studied. Analysis of key proteins of cardiomyocyte metabolism and bioenergetics were associated to heart hemodynamic by the Langendorff System and high-resolution respirometry by Oroboros 2k-Oxygraph during Baseline (CGBL, OGBL) and Ischemia/Reperfusion (CGIR, OGIR) conditions. OG showed a significant increase in total body mass (p<0,0001), epididymal fat (p=0,0018), retroperitoneal fat (p=0,0046) and glycemia (p=0,0471) when compared with CG. Hemodynamic showed lower Max dp/dt (p=0,0007) and heart frequency (BPM) (p=0,0178) in OGIR when compared to OGBL; higher Tau (relaxation time) (p=0,001) in OGIR compared to CGIR and OGLB. Respiratory control ratio (RCR) of isolated heart fibers of OGBL was decreased to fatty acid and carbohydrate oxidation, OGIR only to fatty acid. There were no difference in O©ü consumption of OGBL and OGIR. Hearts from OGBL and OGIR presented lower content of phosphoAKT and IR when compared to CG. We suggested that early life overnutrition induces permanent alterations of cardiac activity and that this process is associated with proteins of the insulin signaling cascade and mitochondrial energy metabolism.
• O2k-Network Lab: BR Rio de Janeiro Moura AS
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Heart
HRR: Oxygraph-2k
