Uribe-Alvarez 2018 Microbiologyopen
|Uribe-Alvarez C, Chiquete-Félix N, Morales-García L, Bohórquez-Hernández A, Delgado-Buenrostro NL, Vaca L, Peña A, Uribe-Carvajal S (2018) Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism. Microbiologyopen [Epub ahead of print].|
Abstract: Wolbachia sp. has colonized over 70% of insect species, successfully manipulating host fertility, protein expression, lifespan, and metabolism. Understanding and engineering the biochemistry and physiology of Wolbachia holds great promise for insect vector-borne disease eradication. Wolbachia is cultured in cell lines, which have long duplication times and are difficult to manipulate and study. The yeast strain Saccharomyces cerevisiae W303 was used successfully as an artificial host for Wolbachia wAlbB. As compared to controls, infected yeast lost viability early, probably as a result of an abnormally high mitochondrial oxidative phosphorylation activity observed at late stages of growth. No respiratory chain proteins from Wolbachia were detected, while several Wolbachia F1F0-ATPase subunits were revealed. After 5 days outside the cell, Wolbachia remained fully infective against insect cells.
• Keywords: Saccharomyces cerevisiae, Wolbachia pipientis, Bioenergetics, Endosymbiosis, Oxidative phosphorylation • Bioblast editor: Plangger M, Kandolf G • O2k-Network Lab: MX Mexico City Uribe-Carvajal S
Labels: MiParea: Respiration Pathology: Infectious
Organism: Saccharomyces cerevisiae
Preparation: Isolated mitochondria
Coupling state: OXPHOS, ET Pathway: N, CIV, NS, ROX HRR: Oxygraph-2k