Thrush 2018 Int J Obes (Lond)

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Thrush AB, Antoun G, Nikpay M, Patten DA, DeVlugt C, Mauger JF, Beauchamp BL, Lau P, Reshke R, Doucet É, Imbeault P, Boushel R, Gibbings D, Hager J, Valsesia A, Slack R, Al-Dirbashi OY, Dent R, McPherson R, Harper ME (2018) Diet-resistant obesity is characterized by a distinct plasma proteomic signature and impaired muscle fiber metabolism. Int J Obes (Lond) 42:353-62.

» PMID: 29151592 Open Access

Thrush AB, Antoun G, Nikpay M, Patten DA, DeVlugt C, Mauger JF, Beauchamp BL, Lau P, Reshke R, Doucet E, Imbeault P, Boushel R, Gibbings D, Hager J, Valsesia A, Slack R, Al-Dirbashi OY, Dent R, McPherson R, Harper ME (2018) Int J Obes (Lond)

Abstract: Inter-individual variability in weight loss during obesity treatment is complex and poorly understood. Here we use whole body and tissue approaches to investigate fuel oxidation characteristics in skeletal muscle fibers, cells, and distinct circulating protein biomarkers before and after a high fat meal (HFM) challenge in those who lost the most (obese diet-sensitive; ODS), vs the least (obese diet-resistant; ODR) amount of weight in a highly controlled weight management program.

In 20 weight stable matched ODS and ODR women who previously completed a standardized clinical weight loss program, we analyzed whole body energetics, and metabolic parameters in vastus lateralis biopsies and plasma samples that were obtained in the fasting state and 6 h after a defined HFM, equivalent to 35% of total daily energy requirements.

At baseline (fasting) and post-HFM, muscle fatty acid oxidation and maximal oxidative phosphorylation were significantly greater in ODS vs ODR, as was reactive oxygen species emission. Plasma proteomics of 1130 proteins pre, and 1, 2, 5 and 6 h after the HFM demonstrated distinct group and interaction differences. Group differences identified s-formyl glutathione hydratase, heat shock 70 kDA protein 1A/B (HSP72), and eukaryotic translation initiation factor 5 (eIF-5) to be higher in ODS vs ODR. Group-time differences included aryl hydrocarbon interacting protein (AIP), peptidylpropyl isomerase D (PPID) and tyrosine protein kinase Fgr, which increased in ODR vs ODS over time. HSP72 levels correlated with muscle oxidation and citrate synthase activity. These proteins circulate in exosomes; exosomes isolated from ODS plasma increased resting, leak and maximal respiration rates in C2C12 myotubes by 58, 21, and 51%, respectively vs those isolated from ODR plasma.

Findings demonstrate distinct muscle metabolism and plasma proteomics in fasting and post-HFM states corresponding in diet-sensitive vs diet-resistant obese women.International Journal of Obesity accepted article preview online, 20 November 2017. doi:10.1038/ijo.2017.286.

Keywords: Obesity, Weight loss, Skeletal muscle, Mitochondria, Exosomes, HSP72 Bioblast editor: Kandolf G O2k-Network Lab: CA Vancouver Boushel RC, CA Ottawa Harper ME


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Obesity 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

Labels, 2018-01