Template:SUIT-003 O2 ce D038

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MitoPedia: SUIT

Steps and respiratory states

Ce1;ce2(Omy);ce3U;ce3Glc;ce3'U;ce4Ama.png

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
(ce2Omy) L(Omy) ce1;(ce2Omy)
  • Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
  • If the aim is to analyse only Crabtree effect, it is recommended to skip this step. However, if there is also interest in studying coupling control, Omy can be added at this point to reach LEAK.
ce3U E ce1;(ce2Omy);ce3U
ce3Glc E ce1;(ce2Omy);ce3U;ce3Glc
ce3'U E ce1;(ce2Omy);ce3U;ce3Glc;ce3'U
  • Noncoupled electron transfer state, ET-state, with ET-capacity, E.
  • Additional uncoupler titrations are performed to ensure reaching ET state after addition of glucose.
ce4Ama ROX ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4Ama
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.


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