Seite 2018 Sci Rep

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Seite S, Mourier A, Camougrand N, Salin B, Figueiredo-Silva AC, Fontagne-Dicharry S, Panserat S, Seiliez I (2018) Dietary methionine deficiency affects oxidative status, mitochondrial integrity and mitophagy in the liver of rainbow trout (Oncorhynchus mykiss). Sci Rep 8:10151.

» PMID: 29977029 Open Access

Seite S, Mourier A, Camougrand N, Salin B, Figueiredo-Silva AC, Fontagne-Dicharry S, Panserat S, Seiliez I (2018) Sci Rep

Abstract: The low levels of methionine in vegetable raw materials represent a limit to their use in aquafeed. Methionine is considered as an important factor in the control of oxidative status. However, restriction of dietary methionine has been shown to reduce generation of mitochondrial oxygen radicals and thus oxidative damage in liver. Here, we aim to evaluate the effect of dietary methionine deficiency in hepatic oxidative status in rainbow trout and identify the underlying mechanisms. Fish were fed for 6 weeks diets containing two different methionine concentrations: deficient (MD, Methionine Deficient diet) or adequate (CTL, control diet). At the end of the experiment, fish fed the MD diet showed a significantly lower body weight and feed efficiency compared to fish fed the CTL diet. Growth reduction of the MD group was associated to a general mitochondrial defect and a concomitant decrease of the oxidative status in the liver. The obtained results also revealed a sharp increase of mitochondrial degradation through mitophagy in these conditions and emphasized the involvement of the PINK1/PARKIN axis in this event. Collectively, these results provide a broader understanding of the mechanisms at play in the reduction of oxidant status upon dietary methionine deficiency.


Bioblast editor: Plangger M, Kandolf G O2k-Network Lab: FR Bordeaux Devin A


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics, nDNA;cell genetics, Exercise physiology;nutrition;life style 


Organism: Fishes  Tissue;cell: Liver  Preparation: Homogenate  Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase  Regulation: Amino acid 

Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k 

Labels, 2018-08