Schiffer 2018 PLoS One

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Schiffer TA, Christensen M, Gustafsson H, Palm F (2018) The effect of inactin on kidney mitochondrial function and production of reactive oxygen species. PLoS One 13:e0207728.

» PMID: 30475856 Open Access

Schiffer TA, Christensen M, Gustafsson H, Palm F (2018) PLoS One

Abstract: Inactin is a long lasting anesthetic agent commonly used in rat studies, but is also shown to exert physiological effects such as reducing renal blood flow, glomerular filtration rate and depressing tubular transport capacity. The effect of inactin on isolated kidney mitochondria is unknown and may be important when studying related topics in anaesthetized animals. The aim of this study was to determine whether inactin exerts effects on mitochondrial function and production of reactive oxygen species. Kidney mitochondrial function and production of reactive oxygen after acutely (5 min) or longer (1.5 hour) anesthetizing rats with inactin was evaluated using high-resolution respirometry. The results demonstrate that inactin significantly improves respiratory control ratio, inhibits complex I in the mitochondrial respiratory chain, reduce both unregulated proton leak and time dependently reduce the regulated proton leak via uncoupling protein-2 and adenine nucleotide translocase. Inactin also contributes to increased mitochondrial hydrogen peroxide production. In conclusion, inactin exerts persistent effects on mitochondrial function and these profound effects on mitochondrial function should to be considered when studying mitochondria isolated from animals anesthesized with inactin.


Bioblast editor: Plangger M O2k-Network Lab: SE Uppsala Liss P


Labels: MiParea: Respiration, Pharmacology;toxicology 

Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Kidney  Preparation: Isolated mitochondria  Enzyme: Uncoupling protein 

Coupling state: LEAK, OXPHOS  Pathway: N, NS  HRR: Oxygraph-2k, O2k-Fluorometer 

Labels, 2018-12, Amplex UltraRed