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SF6847

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SF6847

Description

SF6847 (C18H22N2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF6847 concentrations must be titrated carefully to evaluate the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.

Abbreviation: SF6847

Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta

Application in HRR

SF6847: Tyrphostin A9 or Malonoben (3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenylmethylenepropanedinitrile; C18H22N2O), Sigma-Aldrich: T182, store at RT, CAS: 10537-47-0, M = 282.38 gยทmol-1
Hazard statements: H301, H311; toxic if swallowed, toxic in contact with skin


Preparation of 25 mM stock solution (dissolved in ethanol abs.):
  1. Weigh 5 mg in a dark glass vial, dissolve in 708.3 ยตL ethanol.
  2. Store in dark glass vials at -20 ยฐC.


Preparation of 50 ยตM stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
  1. Mix 998 ยตL ethanol abs. and 2 ยตL of the 25 mM stock solution.
  2. Divide into 0.1 ml aliquotes, use dark glass vials.
  3. Store at -20 ยฐC.
ยป O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 1 ยตL steps of 50 ยตM stock solution using a 10 ยตL Hamilton syringe.
  • Final concentration: 25 nM steps.


Preparation of 12.5 ยตM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
  1. Add 300 ยตL ethanol abs. and 100 ยตL of the 50 ยตM stock solution.
  2. Divide into 0.1 ml aliquots in dark glass vials. Store at -20 ยฐC.
ยป O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (0.5-mL O2k-chamber): 1 ยตL of 12.5 ยตM stock solution using a 10 ยตL Hamilton syringe.
  • Final concentration: 25 nM steps.


Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


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Specific
ยป Artefacts by single dose uncoupling
ยป ATP synthase
ยป CCCP
ยป Coupling-control protocol
ยป DNP
ยป Dyscoupled respiration
ยป FCCP
ยป Is respiration uncoupled - noncoupled - dyscoupled?
ยป Noncoupled respiration: Discussion
ยป Uncoupler
ยป Uncoupled respiration - see ยป Noncoupled respiration
ยป Uncoupling proteins
ยป Uncoupling protein 1
ยป Uncoupler titrations - Optimum uncoupler concentration
Respiratory states and control ratios
ยป Biochemical coupling efficiency
ยป Coupling-control state
ยป Electron-transfer-pathway state
ยป Electron-transfer pathway
E.jpg ET capacity
ยป E-L coupling efficiency
ยป Flux control efficiency
ยป Flux control ratio
ยป LEAK-control ratio
ยป LEAK respiration
ยป Noncoupled respiration
ยป OXPHOS
ยป OXPHOS capacity; ยป State 3
ยป OXPHOS-control ratio, P/E ratio
ยป Respiratory acceptor control ratio
ยป ROUTINE-control ratio
ยป ROUTINE respiration
ยป ROUTINE state
ยป State 3u
ยป State 4
ยป Uncoupling-control ratio UCR
General (alphabetical order)
ยป Adenine nucleotide translocase
ยป Adenylates
ยป Electron transfer pathway
ยป Mitochondrial preparations
ยป mt-membrane potential
ยป Oxygen flux
ยป Phosphorylation system
ยป Proton leak
ยป Proton slip
ยป TIP2k
Other keyword lists
ยป Template:Keywords: Force and membrane potential



MitoPedia topics: Uncoupler