Rocha 2018 J Physiol Biochem

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Rocha C, Koury OH, Scheede-Bergdahl C, Bergdahl A (2018) Cardiac mitochondrial respiration following a low-carbohydrate, high-fat diet in apolipoprotein E-deficient mice. J Physiol Biochem [Epub ahead of print].

» PMID: 30362048

Rocha C, Koury OH, Scheede-Bergdahl C, Bergdahl A (2018) J Physiol Biochem

Abstract: Low-carbohydrate diets are considered to be an effective approach to weight loss and have, subsequently, grown in popularity. Despite the apparent health benefits that these diets may provide for insulin resistance, hypertension, and dyslipidemia, their implications on cardiomyocyte oxidative capacity have yet to be investigated. To evaluate the adaptations induced by a 6-week low-carbohydrate, high-fat (LCHF) diet on mitochondrial respiration, two groups of male mice were investigated: Apolipoprotein E-deficient mice on a LCHF diet (L-DIET) and apolipoprotein E-deficient mice on a regular rodent diet (CON). Heart tissue was extracted and used for high-resolution respirometry (HRR), while immunoblotting was performed to quantify mitochondrial density and complexes. The results demonstrate increased expression of all five mitochondrial subunits in the L-DIET group compared to control condition. Furthermore, HRR revealed increased efficiency of substrate consumption, implying augmented oxidative capacity in the L-DIET group. These findings further support the notion that cardiomyocytes prefer lipids as a primary fuel source, by demonstrating that the shift in metabolism caused by a LCHF diet facilitates such an environment. This provides important information regarding the effects of a LCHF on cardiomyocytes, especially when considering free radical production and heart dysfunction.

Keywords: Apolipoprotein E, Cardiomyocytes, Lipid metabolism, Low-carbohydrate and high-fat diet, Mitochondria, OXPHOS Bioblast editor: Plangger M O2k-Network Lab: CA Montreal Bergdahl A


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Mouse  Tissue;cell: Heart  Preparation: Permeabilized tissue  Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Uncoupling protein 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, CIV, NS, ROX  HRR: Oxygraph-2k 

Labels, 2018-11