Ounpuu 2018 Biochem Cell Biol

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Ounpuu L, Truu L, Shevchuk I, Chekulayev V, Klepinin A, Koit A, Tepp K, Puurand M, Rebane-Klemm E, Käämbre T (2018) Comparative analysis of the bioenergetics of human adenocarcinoma Caco-2 cell line and postoperative tissue samples from colorectal cancer patients. Biochem Cell Biol [Epub ahead of print].

» PMID: 30058357

Ounpuu L, Truu L, Shevchuk I, Chekulayev V, Klepinin A, Koit A, Tepp K, Puurand M, Rebane-Klemm E, Kaeaembre T (2018) Biochem Cell Biol

Abstract: The aim of this work was to explore the key bioenergetic properties attributed to the mitochondrial respiration in widely used Caco-2 cell line and human colorectal cancer (HCC) postoperational tissue samples. Oxygraphy and Metabolic Control Analysis (MCA) were applied to estimate the function of oxidative phosphorylation in cultured Caco-2 cells and HCC tissue samples. The mitochondria of Caco-2 cells and HCC tissues displayed larger functional activity of respiratory complex (C)II compared to CI, whereas in normal colon tissue an inverse pattern in the ratio of CI to CII activity was observed. MCA showed that the respiration in Caco-2 and HCC tissue cells is regulated by different parts of electron transport chain. In HCC tissue, this control is performed essentially at the level of respiratory chain complexes I-IV, whereas in Caco-2 cells at the level of CIV (cytochrome c oxidase) and ATP synthasome. The revealed differences in the regulation of respiratory chain activity and glycose index could represent an adaptive response to distinct growth conditions; this means the importance of proper validation of results obtained from in vitro models before their extrapolation to the more complex in vivo systems.

Keywords: Colorectal cancer, Mitochondrial metabolism, OXPHOS, Metabolic control analysis Bioblast editor: Plangger M, Kandolf G O2k-Network Lab: EE Tallinn Kaambre T


Labels: MiParea: Respiration, Comparative MiP;environmental MiP  Pathology: Cancer 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, Other cell lines  Preparation: Permeabilized cells 


Coupling state: LEAK, OXPHOS  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k 

Labels, 2018-08