Oliveira 2019 Mol Nutr Food Res
|Oliveira TE, Castro É, Belchior T, Andrade ML, Chaves-Filho AB, Peixoto AS, Moreno MF, Ortiz Silva M, Moreira RJ, Inague A, Yoshinaga MY, Miyamoto S, Moustaid-Moussa N, Festuccia WT (2019) Fish oil protects wild type and uncoupling protein 1 deficient mice from obesity and glucose intolerance by increasing energy expenditure. Mol Nutr Food Res [Epub ahead of print].|
Oliveira TE, Castro E, Belchior T, Andrade ML, Chaves-Filho AB, Peixoto AS, Moreno MF, Ortiz Silva M, Moreira RJ, Inague A, Yoshinaga MY, Miyamoto S, Moustaid-Moussa N, Festuccia WT (2019) Mol Nutr Food Res
Abstract: We investigated the mechanisms and involvement of uncoupling protein 1 (UCP1) in the protection from obesity and insulin resistance induced by intake of high-fat diet rich in omega 3 (n-3) fatty acids.
C57BL/6J mice were fed either a low-fat (control group) or one of two isocaloric high-fat diets containing either lard (HFD) or fish oil (HFN3) as fat source and evaluated for body weight, adiposity, energy expenditure, glucose homeostasis and inguinal white and interscapular brown adipose tissues (iWAT and iBAT, respectively) gene expression, lipidome and mitochondrial bioenergetics. HFN3 intake protected from obesity, glucose and insulin intolerances and hyperinsulinemia. This was associated with increased energy expenditure, iWAT UCP1 expression and incorporation of n-3 eicosapentaenoic and docosahexaenoic fatty acids in iWAT and iBAT triacylglycerol. Importantly, HFN3 was equally effective in reducing body weight gain, adiposity and glucose intolerance and increasing energy expenditure in wild type and UCP1-deficient mice without recruiting other thermogenic processes in iWAT and iBAT such as mitochondrial uncoupling, SERCA-mediated calcium and creatine-driven substrate cyclings.
Intake of a high-fat diet rich in omega 3 fatty acids protects both wild type and UCP1-deficient mice from obesity and insulin resistance by increasing energy expenditure through unknown mechanisms.
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• Keywords: Cardiolipin, Energy expenditure, Fish oil, Nonshivering thermogenesis, Obesity, Omega 3 fatty acids, Uncoupling protein 1, White and brown adipose tissues • Bioblast editor: Plangger M • O2k-Network Lab: BR Sao Paulo Festuccia W
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style Pathology: Obesity
Organism: Mouse Tissue;cell: Fat Preparation: Isolated mitochondria Enzyme: Uncoupling protein Regulation: Fatty acid Coupling state: ET Pathway: N HRR: Oxygraph-2k