MitoPathway control state: F
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the FAO-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) FAO-pathway could be overestimated due to a contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration. Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. High concentration of malate, typically 2 mM, saturates the F-pathway.
MitoPedia concepts: SUIT state
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FAO in the LEAK state can be evaluated in the following SUIT protocols:
FAO in the OXPHOS state can be evaluated in the following SUIT protocols:
- DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011
FAO in the ET state can be evaluated in the following SUIT protocols: