Nielsen 2018 Neurosci Lett

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Nielsen B, Cejvanovic V, Wörtwein G, Hansen AR, Marstal KK, Weimann A, Bjerring PN, Dela F, Poulsen HE, Jørgensen MB (2018) Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue. Neurosci Lett [Epub ahead of print].

» PMID: 30287304 Open Access

Nielsen B, Cejvanovic V, Woertwein G, Hansen AR, Marstal KK, Weimann A, Bjerring PN, Dela F, Poulsen HE, Joergensen MB (2018) Neurosci Lett

Abstract: Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H2O2 was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.

Keywords: 8-oxoGuo, Mitochondria, Hydrogen peroxide, Major depression, Reactive oxygen species (ROS) Bioblast editor: Plangger M O2k-Network Lab: DK Copenhagen Dela F, DK Copenhagen Larsen S


Labels: MiParea: Respiration, mt-Medicine  Pathology: Other 

Organism: Rat  Tissue;cell: Heart  Preparation: Homogenate 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

Labels, 2018-10, Amplex UltraRed