Mousovich-Neto 2019 Exp Physiol
|Mousovich-Neto F, Matos MS, Costa ACR, de Melo Reis RA, Atella GC, Miranda-Alves L, Carvalho DP, Ketzer LA, Corrêa da Costa VM (2019) Brown adipose tissue remodeling induced by corticosterone in Wistar male rats. Exp Physiol [Epub ahead of print].|
Abstract: In mammals, brown adipose tissue (BAT) is centrally involved in energy metabolism. To test the hypothesis that glucocorticoids excess disrupts BAT phenotype and function, male Wistar rats were treated with corticosterone in drinking water for 21 days. To confirm induction of glucocorticoid excess and metabolic disturbances, adrenals weight, corticotrophin releasing hormone mRNA levels, corticosterone serum levels, glucose tolerance test and serum triacylglycerol analyses were performed. Adipose tissue deposits were excised, weighed and evaluated by a set of biochemical, histological and molecular procedures, such as: thin-layer chromatography, histochemistry, immunohistochemistry, quantitative real-time polymerase chain reaction, high-resolution oxygraphy, ATP synthesis and enzymatic activity measurements. The approach was successful in induction of glucocorticoid excess and metabolic disturbances. Lower body weight and increased adiposity were observed in corticosterone-treated rats. Interscapular brown adipose tissue (iBAT) showed higher sensitivity to glucocorticoids than other fat deposits. The treatment induced lipid accumulation, unilocular rearrangement, increased collagen content and decreased innervation in iBAT. Furthermore, expression of Prdm16 (P < 0.05), Ucp1 (P < 0.05) and Slc7a10 (P < 0.05) decreased, while expression of Fasn (P < 0.05) and Lep (P < 0.05) mRNA increased in brown adipose tissue. Also, the levels of UCP1 diminished (P < 0.001, 2.5-fold). Finally, lower oxygen consumption (P < 0.05), ATP synthesis (P < 0.05) and mitochondrial content (P < 0.05) were observed in iBAT of GCs-treated rats. Glucocorticoid excess induced an extensive remodeling of interscapular brown adipose tissue, resulting in a white-like phenotype in association with metabolic disturbances. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
• Keywords: Brown adipose tissue, Glucocorticoids, Metabolic disturbances • Bioblast editor: Plangger M
Labels: MiParea: Respiration, nDNA;cell genetics, Exercise physiology;nutrition;life style, Pharmacology;toxicology
Organism: Rat Tissue;cell: Fat Preparation: Isolated mitochondria
Regulation: ATP production Coupling state: OXPHOS Pathway: N, NS HRR: Oxygraph-2k