Menezes-Filho 2018 Biochim Biophys Acta Bioenerg

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Menezes-Filho SL, Amigo I, Luévano-Martínez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35.

» PMID: 30465749

Menezes-Filho SL, Amigo I, Luevano-Martinez LA, Kowaltowski AJ (2018) Biochim Biophys Acta Bioenerg

Abstract: Overnight fasting of rodents is commonly adopted in protocols to obtain isolated liver mitochondria, but the effects of fasting itself on mitochondrial function are poorly characterized. In this study we show that overnight fasting (15 h) promotes a shift in the liver mitochondrial bioenergetic profile, with a reduction in ADP-stimulated and maximal respiration, lower membrane potentials and lower resistance to Ca2+-induced mitochondrial permeability transition. Short term fasting (4 h) promoted similar changes, suggesting that this is a physiological shift in mitochondrial function associated with fasting, but not torpor. Our results suggest that the widely adopted liver mitochondrial isolation technique using fasted animals should be reconsidered, and also uncover physiological changes in bioenergetic function associated to nutritional status.

Keywords: Coupling, Fasting, Liver, Mitochondria, Permeability transition Bioblast editor: Plangger M O2k-Network Lab: BR Sao Paulo Kowaltowski AJ


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Stress:Permeability transition  Organism: Mouse  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Regulation: Calcium, mt-Membrane potential  Coupling state: LEAK, OXPHOS, ET 

HRR: Oxygraph-2k 

Labels, 2018-12