Larsen 2019 Appl Physiol Nutr Metab

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Larsen S, Dandanell S, Kristensen KB, Jørgensen SD, Dela F, Helge JW (2019) Influence of exercise amount and intensity on long-term weight loss maintenance and skeletal muscle mitochondrial ROS production in humans. Appl Physiol Nutr Metab [Epub ahead of print].

» PMID: 30664360

Larsen S, Dandanell S, Kristensen KB, Joergensen SD, Dela F, Helge JW (2019) Appl Physiol Nutr Metab

Abstract: Sustaining a weight loss after a lifestyle intervention is challenging. The objective of the present study was to investigate if mitochondrial function is associated to the ability to maintain a weight loss. 68 former participants in an 11-12-week lifestyle intervention were recruited into two groups; weight loss maintenance (WLM; BMI 32±1 kg/m2) and weight regain (WR; BMI 43±2 kg/m2) based on weight loss measured at a follow-up visit (WLM: 4.8±0.4; WR: 7.6±0.8 years after lifestyle intervention). Maximal oxygen consumption rate (VO2max), physical activity level, blood and muscle samples were obtained at the follow up experiment. Mitochondrial respiratory capacity and reactive oxygen species (ROS) production were measured. Fasting blood samples were used to calculate glucose homeostasis index. WR had an impaired glucose homeostasis a decreased VO2max and physical activity level compared with WLM. The decreased physical activity in WR was due to a lower activity level at vigorous and moderate intensities. Mitochondrial respiratory capacity and citrate synthase (CS) activity was higher in WLM, but intrinsic mitochondrial respiratory capacity (mitochondrial respiratory capacity corrected for mitochondrial content (CS activity)) was similar. ROS production was higher in WR compared with WLM, this was accompanied by a decreased content of antioxidant proteins in WR. Skeletal muscle intrinsic mitochondrial respiratory capacity is not associated with the ability to maintain a long-term weight loss. WLM had a higher VO2max, physical activity level, mitochondrial respiratory capacity and CS activity compared with WR. The reduced glucose tolerance was concurrent with increased ROS production per mitochondria in WR, and could also be associated with the lower physical activity level in this group.

Keywords: Glucose tolerance, Mitochondrial content, Mitochondrial function, Reactive oxygen species production, Skeletal muscle, Weight loss Bioblast editor: Plangger M O2k-Network Lab: DK Copenhagen Dela F, DK Copenhagen Larsen S


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase 

Coupling state: OXPHOS, ET  Pathway: N, NS  HRR: Oxygraph-2k 

Labels, 2019-01