Kahancova 2018 FEBS Lett

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Kahancova A, Sklenar F, Jezek P, Dlaskova A (2018) Regulation of glucose-stimulated insulin secretion by ATPase Inhibitory Factor 1 (IF1). FEBS Lett 592:999-1009.

» PMID: 29380352

Kahancova A, Sklenar F, Jezek P, Dlaskova A (2018) FEBS Lett

Abstract: ATPase Inhibitory factor 1 (IF1) is an endogenous regulator of mitochondrial ATP synthase, which is involved in cellular metabolism. Although great progress has been made, biological roles of IF1 and molecular mechanisms of its action are still to be elucidated. Here, we show that IF1 is present in pancreatic β-cells, bound to the ATP synthase also under normal physiological conditions. IF1 silencing in model pancreatic β-cells (INS-1E) increases insulin secretion over a range of glucose concentrations. The left-shifted dose-response curve reveals excessive insulin secretion even under low glucose, corresponding to fasting conditions. A parallel increase in cellular respiration and ATP levels is observed. To conclude, our results indicate that IF1 is a negative regulator of insulin secretion involved in pancreatic β-cell glucose sensing.

Keywords: ATP synthase, IF1, INS-1E cells, Diabetes, Insulin secretion, β-cells Bioblast editor: Kandolf G O2k-Network Lab: CZ Prague Jezek P


Labels: MiParea: Respiration 


Organism: Rat  Tissue;cell: Other cell lines  Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

Labels, 2018-03