Iyer 2009 Mitochondrion

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Publications in the MiPMap
Iyer S, Thomas R, Portell F, Dunham L, Quigley C, Bennett JP (2009) Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression. Mitochondrion 9:196-203.

» PMID: 19460293 Open Access

Iyer S, Thomas R, Portell F, Dunham L, Quigley C, Bennett JP (2009) Mitochondrion

Abstract: We developed a scalable procedure to produce human mitochondrial transcription factor A (TFAM) modified with an N-terminal protein transduction domain (PTD) and mitochondrial localization signal (MLS) that allow it to cross membranes and enter mitochondria through its “mitochondrial transduction domain” (MTD = PTD + MLS). Alexa488-labeled MTD–TFAM rapidly entered the mitochondrial compartment of cybrid cells carrying the G11778A LHON mutation. MTD–TFAM reversibly increased respiration and levels of respiratory proteins. In vivo treatment of mice with MTD–TFAM increased motor endurance and complex I-driven respiration in mitochondria from brain and skeletal muscle. MTD–TFAM increases mitochondrial bioenergetics and holds promise for treatment of mitochondrial diseases involving deficiencies of energy production.

Keywords: TFAM, Respiration, Mitochondrial gene expression, Respiratory proteins

O2k-Network Lab: US VA Richmond Bennett JP, US VA Richmond Iyer S


Labels: MiParea: Respiration, Genetic knockout;overexpression  Pathology: Inherited 

Organism: Human  Tissue;cell: Skeletal muscle, Nervous system 

Enzyme: Complex I 

Coupling state: ROUTINE 

HRR: Oxygraph-2k