MiPNet14.04 IOC51

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Oroboros Instruments

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MiPNet14.04 IOC51

Publications in the MiPMap
Oroboros Instruments

Schroecken AT, 2009 Apr 22-26. First Workshop on O2k-MultiSensor, IOC51.

» Bioblast pdf

Oroboros (2009-04-22) Mitochondr Physiol Network

Abstract: Demo O2k-Course at MiPsummer 2009. Baton Rouge, USA; 2009 June 21.


O2k-Network Lab: AT_Innsbruck_Oroboros


Labels:






ORO, IOC, 2009 



General information

A first Advanced MultiSensor O2k-Workshop is organized for scientists who have participated previously in one of our introductory O2k-Courses. It will provide an overview on a variety of measurements in addition to O2 in the Oxygraph-2k. The two-chamber design of the O2k offers the possibility to perform simultaneous measurements either in each chamber equipped with ports for additional electrodes or light guides, or in parallel in two different chambers when using larger electrodes that do not fit through the PVDF stopper.


Topics

1.) TPP+ electrode and mitochondrial membrane potential, for meaurements with mitochondrial preparations (isolated mitochondria, permeabilized cells or permeabilized muscle fibres), and other ion selective electrodes.

2.) pH and metabolic acid production (aerobic or anaerobic glycolysis) in intact cells, including ph-stat applications.

3.) NO sensor for measurement of NO dynamics and mitochondrial oxygen kinetics (not in permeabilized tissue preparations with oxygen diffusion limitation).

4.) Oxystat: TIP-2k feedback control of oxygen levels and HRR; measurements at hypoxic steady-states.

5.) Spectrofluorimetry with wide range of applications: HRR and SAFAS Xenius spectrofluorimeter (isolated mitochondria, intact and permeabilized suspended cells).

6.) Spectrophotometry and cytochrome redox states: HRR and LEA Medizintechnik O2c (isolated mitochondria, intact and permeabilized suspended cells).


In addition to these MultiSensor topics, we will present and discuss (depending on specific interests of participants):

  • DatLab 4.3 features for MultiSensor applications and analysis of steady-state experiments.
  • Automatic instrumental background tests with dithionite titrations.
  • Advanced protocols for respiratory OXPHOS control: multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) and enzymatic thresholds.
  • Oxygen kinetics obtained from aerobic-anaerobic transitions and respirometry at steady-state oxygen levels


Lecturers

Erich Gnaiger Gnaiger Erich, CEO, Oroboros Instrumetns and Medical University of Innsbruck, D. Swarovski Research Laboratory

Fasching Mario Fasching Mario, O2k-Network Lab: Oroboros Instrumetns

Harrison David K Harrison David K, O2k-Network Lab: Oroboros Instrumetns


Frank Busotti, O2k-Network Lab: SAFAS, Monaco
Derfuss T, O2k-Network Lab: LEA Medizintechnik
Köfler Simone, O2k-Network Lab: Oroboros Instrumetns


Participants

Participant Institution
Boushel Robert C Boushel Robert C DK Copenhagen Dela F: University of Copenhagen (DK)
Bouillaud Frédéric Bouillaud Frédéric FR Paris Bouillaud F: Faculté de Médecine Necker-Enfants Malades (FR)
Bussotti J. Franck Société Anonyme de Frabricatin d'Appareillages Scientifiques (FR)
Doerrier Velasco Carolina A Doerrier Velasco Carolina A ES Granada Acuna-Castroviejo D: Universidad de Granada (ES)
Fasching Angelica SE Uppsala Liss P: Uppsala University (SE)
Friederich-Persson Malou SE Uppsala Liss P: Uppsala University (SE)
Garcia-Roves Pablo Miguel Garcia-Roves Pablo Miguel SE Stockholm Morein T: Karolinska Institute (SE)
Gradl Lukas AT Innsbruck Oroboros (AT)
Hickey Anthony J Hickey Anthony J NZ Auckland Hickey AJ: University of Auckland (NZ)
Kane DA.jpg Kane Daniel A CA Antigonish Kane DA: St. Francis Xavier University (CA)
Kane Constance L CA Antigonish Kane DA: St. Francis Xavier University (CA)
Kraunsoee Regitze DK Copenhagen Dela F: University of Copenhagen (DK)
Lancel Steve FR Lille Neviere R: Univ. Droit et Sante - Lille II (FR)
Hey-Mogensen Martin DK Copenhagen Dela F: University of Copenhagen (DK)
Perry Christopher G Perry Christopher G CA Toronto Perry CG: York University (CA)
Stride Nis DK Copenhagen Dela F: University of Copenhagen (DK)
Thouas George AU Melbourne Thouas G: University of Melbourne (AU)
Wang Xiaoyang Gothenburg University (SE)
Wessels Bart NL Eindhoven Nicolay K: Eindhoven University of Technology (NL)


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O2k-Workshops are listed as MitoGlobal Events.