Galvan 2019 Biochimie

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Publications in the MiPMap
Galván AE, Chalón MC, Ríos Colombo NS, Schurig-Briccio LA, Sosa-Padilla B, Gennis RB, Bellomio A (2019) Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli. Biochimie [Epub ahead of print].

» PMID: 30790617

Galvan AE, Chalon MC, Rios Colombo NS, Schurig-Briccio LA, Sosa-Padilla B, Gennis RB, Bellomio A (2019) Biochimie

Abstract: Microcin J25 (MccJ25), an antimicrobial peptide, targets the respiratory chain but the exact mechanism by which it does so remains unclear. Here, we reveal that MccJ25 is able to inhibit the enzymatic activity of the isolated cytochrome bd-I from E. coli and induces at the same time production of reactive oxygen species. MccJ25 behaves as a dose-dependent weak inhibitor. Intriguingly, MccJ25 is capable of producing a change in the oxidation state of cytochrome bd-I causing its partial reduction in the presence of cyanide. These effects are specific for cytochrome bd-I, since the peptide is not able to act on purified cytochrome bo3.

Copyright © 2019 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Keywords: ROS, Redox peptide, Cytochrome bd-I, Respiratory chain Bioblast editor: Plangger M


Labels: MiParea: Respiration, Pharmacology;toxicology 

Stress:Oxidative stress;RONS  Organism: Eubacteria 

Preparation: Oxidase;biochemical oxidation 

Regulation: Redox state, Substrate 


HRR: Oxygraph-2k 

Labels, 2019-02